STUDY OF ANTHELMINTIC AND ANTIMICROBIAL ACTIVITY OF PEEL EXTRACT OF PUNICA GRANATUM LINN.

Deore Leena P*., Bachhav Devidas G., Nikam Vikas K., Heda Amol J.

ABSTRACT

Punica granatum linn. (pomegranate) has been used for centuries to confer health benefits in number of diseases . The aim of present study is to identify phytochemical constituents and evaluation of antimicrobial and anthelmintic activities of peel extract of pomegranate. Antimicrobial activity of plant is due to its secondary metabolites. The antimicrobial activity of peel extract in aqueous, ethanol and methanol on E.coli, S.aureus, and B.subtilis was studied at different concentrations (1, 5, 10 mg/ ml). E. coli shows significant zone of inhibition in ethanol extract and S.aureus in aqueous extract, while B. subtilis shows in methanolic extract.The various concentrations (20, 50, 100, and 150 mg/ml) of aqueous, methanol and ethanol extracts were tested in-vitro for anthelmintic potency. It was determined by time required for paralysis and time required for death of earth worms. The peel extract shows dose dependant activity .The short time for paralysis and death was observed for methanol extract at concentration 150 mg/ml. From above observations it was concluded that the methanolic extract shows maximum response in dose dependant manner for antimicrobial and anthelmintic activities.

Keywords: Punica granatum linn., Pomegranate, Anthelmintic, Antimicrobial.