DIVERSE BIOMOLECULES PRESENT IN ETHANOLIC EXTRACT OF FRUITS OF PUNICA GRANATUM L(EEF-PG) ACT AS POTENTIAL HEPATOCYTES REGENERATOR

Asish Bhaumik*, SK. Nagulmeera, G. Neelamma, Sanjoy Das and K. Sreelatha

ABSTRACT

Punica granatum is grown as a fruit crop plant, and as ornamental trees and shrubs in parks and gardens. The edible fruit of Punica granatum is a berry and is between a lemon and a grapefruit in size, 5–12 cm in diameter with a rounded hexagonal shape and has thick reddish skin. The main aim and objectives of the present work was to isolate the bioactive molecules from the ethanolic extract of fruits Punica granatum (EEF-PG) and evaluate the in vivo hepatoprotective activity. The isolationation and characterization of biomolecules was carried out by ESI-MS-MS. In vivo hepatoprotective activity was performed against CCl4 induced rat hepatocytes. The Results displayed that the elevated levels of SGOT, SGPT, ALP and Serum bilirubin due to CCl4 intoxication were reduced significantly (*P<0.05) in rats, after treatment with EEF-PG. Treatment with EEF-PG at a both doses of 200 and 400 mg/kg b.w. significantly decreased the SGOT, SGPT, ALP, Serum Bilirubin levels by 10.76%, 23.30%, 10.18% and 36.98% (at low dose) and 25.33%, 42.26%, 22.66% and 54.79% (at high dose) respectively. Silymarin used as standard drug showed a reduction of 74.19%, 76.91%, 86.7% and 78.08% receiving CCl4 alone. So depending upon the experimental evidence, it was confirmed that the biochemical parameters of the group treated with EEF-PG was significantly lower than the CCl4-treated group. Histopathological examination of liver section of the rat treated with both 200 mg/kg, 400 mg/kg body weight of EEF-PG intoxicated with carbon tetrachloride had shown minimal inflammation with moderate portal triditis, moderate necrotic debris, centrilobular regeneration with restoration of central vein, sinusoids and hepatocytes with mild necrosis and their lobular architecture was normal.

Keywords: Bioactive molecules, Hepatoprotective activity, ESI-MS-MS, Portal triditis, centrilobular regeneration etc.