SIMULTANEOUS ESTIMATION OF VILDAGLIPTIN AND METFORMIN BY SIMULTANEOUSEQUATION METHOD IN BULK AND COMBINED TABLET FORMULATION USING SPECTROPHOTOMETRY

Khagga Bhavya Sri*, Saba Fatima, B. Anila and M. Sumakanth

ABSTRACT

A simple, rapid and economical UV-spectroscopic method was developedfor the simultaneous estimation of Vildagliptin and Metformin in pure form and combined tablet dosage form. This method involved use of simultaneous equation, where the concentration of drugs was determined by using absorptivity values of pure drugs Metformin andVildagliptin, along with the absorbance values of the combined tablet dosage form at selected wavelengths of 233nm and 266nm. Metformin is an anti-diabetic drug belonging to the class of biguanides and is used as the first-line medication. Vildagliptin is an oral anti-diabetic drug which belongs to class of drugs which inhibitdipeptidyl peptidase-4 (DPP-4) enzyme. The Vildagliptin and Metformin in combination has been used in diabetic patients to reduce the glycaemic index which has better results than the mono-drug therapy. The diluents distilled water and double distilled water were used for Metformin and Vildagliptin pure drugs respectively. For the analysis of drugs in combined tablet dosage form, double distilled water was used as a diluent. The method developed was easy to carry outand can be used as a routine quality control test for simultaneous estimation of combined tablet dosage form.

Keywords: Simultaneous equation, Vildagliptin, Metformin, combined tablet dosage form.