A REVIEW ON COMPARISON BETWEEN NORMAL PHASE AND REVERE PHASE HPLC

Priyanka*, Dr. Mukesh Bansal and Dr. Dilip Agarwal

ABSTRACT

This review mainly focuses on comparing between normal phase and reverse phase HPLC also its instrumentation and applications. The importance of RPHPLC in analytical method development and their strategies along with brief knowledge of critical chromatographic parameters needed for optimized an efficient method development has been mentioned. The key difference between reverse phase and normal phase HPLC is that the reverse phase HPLC uses a nonpolar stationary phase and a polar mobile phase whereas the normal phase HPLC uses a polar stationary phase and a less polar mobile phase.

Keywords: Chromatography, RPHPLC, Instrumentation of HPLC, Applications.