FORMULATION & EVALUATION OF GENISTEIN NANOEMULSION FOR ENHANCED TOPICAL DELIVERY

Dr. Preeti Karwa*, Ayesha Syed, Priyanka Priyadarshini

ABSTRACT

Purpose: Genistein is an isoflavone compound having poor water solubility and high membrane permeability. The oral delivery is frequently associated with low bioavailability, high intra- and inter-subject variability and therapeutic failure. Hence an attempt was made by designing the Nanoemulsion (NE) systems through topical application in order to achieve an enhancement in solubility and faster onset of action. Methods: Equilibrium solubility studies indicated the choice of paraffin liquid as oily phase, Tween 20 and PEG 400 as emulgents for formulating the NE. From the ternary phase diagram, the surfactant and co-surfactant ratio was selected as 3:1. The NE was systematically optimized by 32 full factorial design. The oil concentration (X1) and Oil: Smix ratios (X2) were selected as independent variables and Droplet size (Y1), Emulsification time (Y2), Polydispersity index (PDI) (Y3) and Entrapment Efficiency (Y4) were selected as dependent variables. Results: The optimized formulation F1 showed droplet size 19.46 nm, PDI 0.242, emulsification time 31.7 sec, EE of 94.64% and in-vitro release of 80.8% in 12 hrs. Also, the zeta potential was found to be -28.42mV which indicated Formulation F1 was stable. The nanometer size range and the anti-oxidant activity of the optimized F1 formulation was found to be high enough to neutralize the free radicals. Conclusion: Hence, it can be concluded that NE formulation of genistein improved the dissolution of genistein with enhanced anti oxidant property.

Keywords: Nanoemulsion (NE), Genistein, Emulgents, full factorial designs, PDI.