EFFECT OF GROWTH FACTORS ON HYDROGEN PRODUCTION BY RHODOPSUEDOMONAS PALUSTRIS KU 001 AND RHODOPSUEDOMONAS PALUSTRIS OU 001

Z. Anjana, *Karunakar Rao Kudle, Koyatti. Rama, D. R. Manisha, Madhukar Rao Kudle, M. P. Pratap Rudra*

ABSTRACT

Anoxygenic photosynthetic bacteria Rhodopsuedomonas palustris KU 001 and Rhodopsuedomonas palustris OU 001 were isolated and the hydrogen production abilities were analysed in the present study. Studies on optimization of cultural conditions for production of hydrogen revealed that the maximum hydrogen production took Place at 48th hours of incubation in both the strains isolated. More hydrogen production was seen when biotin, thiamine and Cyanocobalamine were used as growth factor sources for R.palustris KU001 while high hydrogen production was seen in thiamine, cyanocobalamine followed by biotin in R.palustris OU001. pH of 6.8 was amenable for the production of hydrogen and high biomass, hydrogen production two Rhodopseudomonas palustris KU001 and Rhodopseudomonas palustris OU001.

Keywords: Hydrogen production, R.palustris, sewage water, growth factors.