PIEZOELECTRIC MATERIALS FOR SONODYNAMIC THERAPY IN TUMOUR TREATMENT

Attah Idris Mamudu, Sandeep Kumar Shukla and Ibrahim Aminu Shehu*

ABSTRACT

The conventional therapy of cancer has been replaced with non-invasive approaches, among which sonodynamic therapy (SDT) emerged as a golden technique that effectively destroys cancerous cells. It expedites the production of reactive oxygen species (ROS) intracellularly allowing the high specificity in tumour cell destruction. In this study, we use BCTZO piezogel as piezoelectric materials in SDT on MCF-7 cells. Methodology: The MCF-7 cells were treated using three different concentrations (8, 16 and 32 μg/mL) of BCTZO and BCZTO-Ag for 48 h. Hoechst 33342, which is a specific strain used for AT-rich regions of double-stranded DNA was utilized. The cells were incubated for 15 min with Hoechst 33342 dye (5 μg/mL in PBS) and subsequently visualized using a BHZ, RFCA microscope equipped with a fluorescent light source with an excitation wavelength of 330 nm and a barrier filter of 420 nm. Conclusion: Outcomes of Hoechst staining carious out on MCF-7 cells indicated that the BCZTO-Ag US has the highest apoptotic effect when compared with the cell treated with BCZTO, US and control line respectively. Therefore, BCZTO-Ag US could be the best therapeutic approach for cancer chemotherapy.

Keywords: Sonodynamic therapy, Piezoelectric materials, MCF-7 cells, BCZTO-Ag US.