EVALUATION FOR ANTI-PYRETIC, ANALGESIC AND ANTI-INFLAMMATORY ACTIVITY OF SYNTHETIC PIPERONAL DERIVATIVES

A. Mamatha*, K. Venkateshwarlu and Ch. Suresh

ABSTRACT

Recent studies have revealed the key roles of pro-inflammatory cytokines, such as tumor necrosis factor-a (TNF-a), interleukin-1b (IL-1b), IL-6 and IL-8 in the pathogenesis of RA.[20] In the present study, we showed that petroleum ether, ethyl acetate and methanolic extract at a dose of 50 and 100 mg/kg body weight significantly inhibit the progression of the rheumatoid arthritis in treated animals. The effect of the extracts was dose dependent and for a long period compared to the standard. Earlier observations by Rekha et al (2010) supported the alterations in the metabolic activities of diseased rats.[21] Earlier findings suggest that absorption of 14C-glucose and 14C-leucine in rat’s intestine was reduced in inflamed rats and it shows that the anti-inflammatory drugs have corrected the decreased absorption capacity of intestine during inflammation.[22,23] The increased body weight during the treatment with diclofenac and the extracts of Cassia uniflora leaves observed in this work may be due to the restoration of the absorption capacity of the intestine. Objective: To evaluate the anti-pyretic, anti-inflammatory and analgesic activity of synthetic Piperonal derivatives. Method: The Piperonal derivatives were administered orally to animals at 100mg/kg dose. Anti-inflammatory activity was studied in male Wistar rats using carrageenan induced paw edema method. Analgesic activity was evaluated in mice by using hot plate method. Antipyretic activity was evaluated by using brewer’s yeast induced pyrexia method. Results: The study showed that the piperonal derivatives were having anti-inflammatory activity at the 100mg/kg test doses. The analgesic activity was also found to significant (p<0.05) and dose dependent prolongation of response latency in the hot plate test. The antipyretic assay revealed the temperature decreasing pattern significantly (p<0.01) when compared with control. Conclusion: These results demonstrated that Piperonal derivatives have potential health benefits as it can produce significant anti-inflammatory, analgesic and anti-pyretic activity.

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