PHYTOCHEMICAL AND INVITRO ANTIOXIDANT ACTIVITY OF AQUEOUS EXTRACT OF NYCTANTHES ARBOR-TRISTIS (L) FLOWER

S. Sumathi* and S. Sangavi

ABSTRACT

Objective: The objective of present work is to study the phytochemical analysis and in vitro antioxidant activity of aqueous extract of Nyctanthes arbor-tristis(L) flower and to find out the method having highest antioxidant activity. Materials and methods: Phytochemical analysis and the in vitro anti-oxidant activity of the aqueous extract were studied using 1,1-diphenyl-2-picryl hydrazyl (DPPH) radical scavenging activity, hydrogen peroxide scavenging activity and total antioxidant activity. Result: The preliminary phytochemical analysis was done to find out the presence of various bioactive compounds such as flavonoids, glycosides, tannins, terpenoids, saponins, resins, carbohydrate, phlobatannins, antheroquinones. Besides it is also possess strong antioxidant activity. Aqueous extract of flowers was studied for its invitro antioxidant activity with different methods viz DPPH, hydrogen peroxide and total antioxidant activity. Conclusion: It was concluded that Nyctanthes arbor-tristis (L) flower possessed a wide range of pharmacologically important phytoconstituents which exhibited strong antioxidant activity in the method of DPPH radical scavenging activity.

Keywords: Phytochemical, Antioxidant, Aqueous extract.