SIMULTANEOUS IDENTIFICATION OF GALLIC ACID, WITHAFERIN A, PIPERINE AND SPIRULINA BY HPTLC FOR STANDARDIZATION OF MULTICOMPONENT IMMUNITY BOOSTER CAPSULE

Unmesh Keshwar*, Deepmala Matey, Soumit Pimplapure, Sharad Dhurde and B. K. Shrikhande

ABSTRACT

Background: Standardization of multicomponent herbal formulation with respect to its active marker compound is still a major challenge. Siddhayu Ayurvedic Research Foundation Pvt. Ltd. has standardized multicomponent herbal capsule formulation with respect to its active marker compounds. Objective: Objective of the present study was to standardize multicomponent herbal capsule formulation by evaluating identification of its active marker compounds simultaneously, purity and impurity. Methods: The standardization parameters such as organoleptic evaluation, Simultaneous identification of active marker compounds by HPTLC, Average weight, Uniformity of weight, Disintegration, Assay (Protein content), Heavy Metals, Microbiological tests for the multicomponent herbal capsule formulation were done. Simultaneous chromatographic separation was achieved by using Toluene: Ethyl Acetate: Methanol: Glacial acetic acid, 5.0: 2.5: 0.5: 0.5 (v/v/v/v) as mobile phase followed by comparing the Rf values and overlaying the spectra with standards. The method was validated according to International Conference on Harmonization guidelines. Results: The presence of active marker compounds Gallic acid, Withaferin A and Piperine in multicomponent herbal capsule formulation was confirmed simultaneously by comparing the Rf values and overlaying the spectra with standards. Identification of Spirulina was confirmed in capsule by the fingerprint profile comparisons with that of reference Spirulina. All the test results of the formulated capsule evaluated were found well within the limits. Conclusion: The developed identification method by HPTLC was found to be simple, rapid, cost effective and able to identify the active marker compounds simultaneously, hence can be routinely employed for analysis in Quality Control Lab.

Keywords: Gallic acid, Withaferin A, Piperine, Spirulina, multicomponent immunity booster capsule, HPTLC.