QUANTITATIVE EVALUATION OF BIOFILM FORMATION ON IMPLANT SURFACE AFTER THREE DIFFERENT MECHANICAL DEBRIDEMENT METHODS: AN IN VITRO MICROBIOLOGICAL STUDY

*Dr. Akshada Gandhi, Dr. Janak Kapadia, Dr. Surekha Bhedasgaonkar, Dr. Ravikumar Jirali, Dr. Harsha Waswade and Dr. Kishore Bhat

ABSTRACT

Like teeth, the dental implant is surrounded by soft and hard tissues. These tissues are also prone to infection and are called peri-implant diseases. The peri-implant diseases resemble periodontal diseases that they are both initiated by plaque formation. The plaque control on the implant surface is thus necessitated for control of the biofilm formation and resultant adverse effects. The present study aims to evaluate and compare the difference in biofilm formation on implant surface after different mechanical debridement methods. Of total 20 implants, 5 implants underwent instrumentation using stainless steel scaler (Hu Friedy 204S Stainless steel sickle scaler), 5 implants underwent instrumentation using plastic scaler (Hu Friedy IMPL 204S IMPLACARE II, 5 implants underwent instrumentation using titanium scaler (Hu Friedy IMPL 204SDT Titanium Scaler) and 5 implants were left uninstrumented. The implant surfaces were irrigated using sterile normal saline in a syringe to clear off all the scrapings if generated and then visualized under the stereomicroscope at 40x magnification to evaluate for surface changes. Four organisms were used for the in vitro biofilm formation in the study - Streptococcus mitis, Streptococcus oralis, Porphyromonas gingivalis and Fusobacterium nucleatum. At the end of incubation period of 4 weeks, the quantification of the bacteria was carried out in terms of optical density and colony forming units. The findings of the present study show that in spite of different degrees of alterations caused by different instruments, there is no statistically significant difference in the bacterial colonization with different treatment modalities. The study suggests that instrumentation types are safe and non-damaging and can be implemented as needed for mechanical debridement.

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