INVITRO CYTOTOXIC ACTIVITY OF GLYCOSIDES FROM LEAVES AND SEEDS OF CORCHORUS SPECIES AGAINST K-562 AND VERO CELL LINES

Carol P. Macwan*, N. M. Patel, B. N. Suhagia, Vipul P. Patel, Mayuree A. Patel

ABSTRACT

The study was aimed to evaluation of the antileukaemic activity of the leaves and seeds of Corchorus aestuans L., Corchorus olitorius L. and Corchorus trilocularis L. on the K-562 cell line. The methanolic extract of Corchorus species have been screened for antileukaemic potentials using in vitro assay for Cytotoxic activity (MTT assay) with ten different concentrations. Measurements were performed and the concentration required for a 50% inhibition of viability (IC50) was determined graphically. The percentage viability of the cell line was carried out by using Trypan blue dye exclusion method. The concentration causing 50% cell growth inhibition (IC50) was determined from DRC using Graph Pad Prism software. Corchorus aestuans L. seed (IC50 value 37.09μg/ml, R2=0.9930) and Corchorus trilocularis L. leaf (IC50 value 67.44μg/ml, R2=0.9952) showed good cytotoxicity activity as compare to Std. drug (Colchicin) having IC50 value 105.5 μg/ml, R2=0.9950).

Keywords: Cytotoxicity, K-562, Corchorus aestuans L., Corchorus olitorius L., Corchorus trilocularis L.