FUNCTIONAL ANNOTATION AND STRUCTURAL CHARACTERISATION OF OPSIN PROTEIN IN LEPTUCA PUGILATOR

S. Harshini and Dr. K. Shoba*

ABSTRACT

Fiddler crabs, which belong to the Ocypodidae family and subfamily Ucinae, are prevalent across the world's tropics and subtropics. Decapod crustaceans in the Uca family include Leptuca pugilator(Fiddler crabs), often known as the Atlantic sand fiddler crab or calling crab. They are brachyuran crabs from the Ocypodidae family, which are among the most recent aquatic organisms to reach the shore. Fiddler crabs are easily recognised by their distinctly asymmetrical claws, and they can be found in the Algarve regions of Portugal, West Africa, the Western Atlantic, the Eastern Pacific, and the Indo-Pacific. These species are present in seacoasts and brackish intertidal mud flats, lagoons and swamps, mangroves, salt marshes, and sandy or muddy coasts. Opsin, a photoreceptor protein found in fiddler crab species, functions as a fundamental piece of molecular machinery for colour perception and maybe trichromatic vision. The Protein and Nucleotide sequence of opsin protein from Leptuca pugilator were retrieved using NCBI database in fasta format. The Functional analysis were done through using AmiGO and T-COFFEE followed by Gene Expression analysis using JCAT Server. The Structural and Biophysical Characterization done through using SWISS-MODEL and Dipole Moment Server. Furthermore, our study investigates which shows the different insilico analysis of the Opsin protein in Leptuca pugilator which will increase the Opsin protein characteristics for the range of various medicinal applications.

Keywords: Bioinformatics; Leptuca pugilator; Opsin; Photoreceptor protein; Functional Analysis; Structural Analysis; Medicinal applications.