EVALUATION OF ANTI-INFLAMMATORY ACTIVITY OF THE EXTRACT OF MADHUCA INDICA USING IN VITRO AND IN VIVO MODELS OF INFLAMMATION

Anoop Prajapati*, Deepak Basedia, Vivek Thakur and Balkrishna Dubey

ABSTRACT

Madhuca indica flower and seeds have been found to contain several phytoconsitutents. In the present investigation, the bark of Madhuca indica was extracted using various solvents and the anti-inflammatory potential of the extract was investigated. The extraction abilities of different solvents for recovering extractable components from leaves followed the order: ethyl acetate>ethanol>water>n-hexane. The phenolic content was found to be highest in the ethyl acetate extract (59.57±3.566 %w/w) followed by ethanol (41.5±1.804 %w/w) and aqueous (20.87±1.054 %w/w) extracts. The ethyl acetate extract of the plant (EAEx) was subjected to in vitro and in vivo determination of anti-inflammatory potential. Inhibition of albumin denaturation was used as the in vitro model while carrageenan induce rat paw edema was used as the in vivo model for anti-inflammatory activity. The ethyl acetate extract of Madhuca indica exhibited the inhibition of albumin denaturation at all doses from (100-500 μg/ml) in a dose dependent manner. The 500 μg/mL concentration of the extract had shown the greatest inhibition capacity (39.6±3.869%) whereas the lowest inhibition capacity was exhibited by concentration 100 μg/mL (7.5±2.291%). EAEx at dose level of 200 mg/Kg exhibited maximum reduction in edema after 4h with 23.98% reduction whereas the change decreased at the 4th hour to 12.95%. EAEx dose of 400 mg/Kg was indeed able to exhibit better reduction in edema as compared to the control with 37.28% reduction after 3h. The reduction decreased to 36.44% after the 4th hour. The effect of EAEx was therefore found to be short acting.

Keywords: Madhuca indica, albumin denaturation, carrageenan, edema, inflammation.