EUROPEAN JOURNAL OF
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Abstract

HPTLC FINGERPRINTING ANALYSIS OF THE ETHANOLIC EXTRACT OF BAUHINIA RACEMOSA LEAVES

Dr. P. Anitha*, E. Ravichandhiran and Rubathi Krishnan P.

ABSTRACT

Objective: To establish the fingerprint profile of Bauhinia racemosa leaves using high performance thin layer chromatography (HPTLC) technique. Methods: Preliminary phytochemical screening was done and HPTLC studies were carried out. CAMAG HPTLC system equipped with Linomat V applicator, TLC scanner 3, Reprostar 3 and WIN CATS1.3.4 software was used. Results: HPTLC finger printing of ethanol extract of leaf revealed 14 peaks with Rf values in the range of 0.02 to 0.85 with flavonoid compound compared to Rf value of standard quercetin Conclusions: It can be concluded that HPTLC fingerprint analysis of leaf extract of Bauhinia racemosa are used in quality assessment and identification of Phyto marker compounds. By isolating and distinguishing the biomarker compounds, novel drugs can be produced to treat various diseases.

Keywords: Bauhinia racemosa, HPTLC fingerprinting, flavonoids.


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SJIF Impact Factor: 7.065

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