UV SPECTROPHOTOMETRIC METHOD DEVELOPMENT AND VALIDATION FOR THE ESTIMATION OF TOPIROXOSTAT IN BULK AND PHARMACEUTICAL DOSAGE FORM

Pallavi Suthar* and Manali Ram

ABSTRACT

A simple, sensitive, rapid, accurate, reproducible and less time consuming UV visible spectroscopic method has been developed for the determination of topiroxostat in bulk and tablet dosage form. The wavelength maxima for topiroxostat was found to be 320.0nm. Beer’s law was obeyed in the concentration range of 10-50μg/ml. The limit of detection (LOD) and limit of quantification (LOQ) were found to be 3μg/ml and 9.27μg/ml. The percentage recovery of the drug for the proposed method was 99.63% indicating no interference of tablet excipients. The results demonstrate that proposed method is accurate, precise and reproducible while being simple and rapid too for determination of topiroxostat in tablet dosage form.

Keywords: Validation, Range, Concentration, Method development, Percentage recovery.