FORMULATION AND STANDARDISATION OF ANTIMICROBIAL HAND WASH FROM PIPER BETLE LEAF EXTRACT

Shafna Hussain*, Ajmal Karuvanthody, Asna M., Huda Mariyam P. K., Nafla K. and Sifna V. U., Dr. Celestin Baboo R. V. and Dr. Sirajudheen M. K.

ABSTRACT

Hand hygiene plays a critical role in preventing the transmission of infectious diseases. This study focuses on the formulation and standardization of an antimicrobial hand wash using Piper betle leaf extract. The Piper betle plant, commonly known as betel leaf, possesses various bioactive compounds with antimicrobial properties. The leaf extract was prepared using the Soxhlet extraction method with a suitable solvent, such as methanol, and its antibacterial activity was assessed using the agar well plate diffusion method with Staphylococcus aureus. The formulation of the antimicrobial hand wash involved incorporating the Piper betle leaf extract into a suitable base formulation comprising surfactants, emollients, and preservatives. Physicochemical parameters such as pH, viscosity, and stability were also assessed to ensure product quality and shelf-life. Gram-positive organism were significantly inhibited by the standardised anti microbial hand wash composition.the hand wash showed good stability and skin compatibility. Though the result showed that formulation and standardisation of an antimicrobial hand wash from Piper betle leaf extract offer a promising approach to developing a natural and effective product for hand hygiene. This research contributes to the growing field of natural product-based antimicrobial formulations and highlights the potential of Piper betle as a valuable source for developing novel antimicrobial agents.

Keywords: Piper betle leaf, leaves extract, hand wash, agar well plate diffusion method.