THE SYSTEMIC ANTICANCER REPROGRAMMING EFFECT OF VITAMIN E ON DU145 PROSTATE CELL LINE

Shvachko L. P.*, Dybkov M. V. and Telegeev G. D.

ABSTRACT

DU145 is a prostate cancer cell line with an origin epithelial morphology. Therefore, the goal was to show that DU145 cells can be modulated in the degree of malignancy of the corresponding prostate cancer cells. It was revealed that Vitamin E (alpha-tocopherol) observed modulating functions on DU145 cancer prostate cells. By the method of qRT-PCR was detected the targeted vitamin E-dependent decreasing mRNA expression levels of specific regulation genes of cancer-associated oxidative stress as thioredoxin reductase (TXN2) and glutathione peroxidase (GPX4) genes. This is the first modulating as antioxidant vitamin E function. The second modulating function of vitamin E was studied as reversed stemness EMT by repression SNAIL EMT-inducer transcription factor and re-activation of the epithelial marker E-cadherin. The third modulating function of vitamin E was studied as remodeling pluripotency vitamin E function was detected on DU145 cancer prostate cells during vitamin E-dependent repression of Oklt4 pluripotency gene mRNA expression and reactivation of C/EBP alpha differentiation transcription factor. The present research emphasized the systemic anticancer function of Vitamin E (alpha-tocopherol) in Reprogramming of cancer-associated phenotype in DU145 cancer prostate cell line investigation.

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