GREEN SYNTHESIS OF SILVER NANOPARTICLES FROM RHYNCHOSIA HEYNEI PLANT LEAF EXTRACT: CHARACTERIZATION, ANTIMICROBIAL, ANTIOXIDANT, AND BIOCATALYTIC PROPERTIES.

Nagendra Potladhurthi and Narasimha Golla*

ABSTRACT

Objective: To study the green synthesis of silver Nanoparticles (AgNps) from plant leaf of Rhynchosia heynei, for the characterization of AgNPs by UV, zeta potential, FTIR and SEM techniques and to study the antioxidant, antimicrobial, and biocatalytic properties. Methods: the Rhynchosia heynei leaf extract was used for the green synthesis of AgNPs. The antioxidant, antibacterial, antifungal activity, biocatalytic activity and characterisation of Nanoparticles with UV- Vis Spectrophotometer, Zeta potential, FTIR, and SEM. Results: the plant leaf of Rhynchosia heynei has shown good antioxidant activity, also shown good antibacterial activity against Gram positive bacteria (Bacillus substilis) and Gram negative bacteria(Pseudomonas auregenosa). The leaf also has good antifungal activity against Aspergillus niger. The leaf also shows good bio catalytic activity of Brilliant green 16% and Bromothymol 18%. The UV absorbance is at 450nm. The size and shape of AgNps (30-120nm) were confirmed by scanning electron microscopy (SEM) studies. Conclusions: The synthesized AgNps shows very good antibacterial antifungal and antioxidant properties.

Keywords: Rhynehosia heynei, Silver Nanoparticles, Characterization. Antioxidant, Antimicrobial and Bio-catalytic properties.