THE PREDICTIVE ROLE OF GENE EXPRESSION LEVEL (LNCRNA HOTAIR) IN THE THERAPEUTIC RESPONSE TO NEOADJUVANT CHEMOTHERAPY IN BREAST CANCER PATIENTS

Ansam Abbdullah*, Faisal Redwan, Nader Abdullaa and Eyad Alshatty

ABSTRACT

Background: Neoadjuvant chemotherapy is considered before therapeutic strategies in early-staged tumors to reduce size, lymph node metastases and improving the chance of performing conserrative breast tissue surgery. The role of Long non-coding RNA LncRNA molecules has been studied in breast cancer. The HOTAIR molecules is considered one of the first molecules studies, as several experimental studies have clarified its role in disease mechanisms due to the occurrence of hyper-proliferation local invasion tumor, metastasis, and lack of response to pharmaceutical treatment. Our study aims to evaluate the gene expression level of HOTAIR molecule in the plasma and its relationship with complete tissue response to treatment. Materials and Methods: An assay was performed for the level of the HOTAIR molecule in the plasma of 30 patients by performing a reverse transcription reaction qPCR. Gene expressional level was evaluated by using LIVAK method The occurrence of a complete histological response was determined through the histology report after surgery. Then a statistical analysis is performed. Results: The results showed significant difference (0.005 > p) between the level of gene expression of HOTAIR molecule and the Occurrence of a complete tissue response to treatment.

Keywords: Breast Cancer, HOTAIR, neoadjuvant therapy, LncRNA.