EFFECT OF TREMA GUINEENSIS AQUEOUS AND ETHANOLIC EXTRACTS ON IRON, COPPER, ZINC AND MANGANESE DURING THE ACUTE INFLAMMATION AND OXIDATIVE STRESS IN RAT.

Kouakou Yeboue Koffi F.*, Yapo Crezoit Claire A., Yapi Houphouet Félix, Gnahoue Goueh, Djaman Allico J.

ABSTRACT

The aim of this study was to investigate in vivo effects of Trema guineensis extracts (TGE) leaves on traces element (Fe, Cu, Mn, Zn) concentrations in carrageenan induced in rats. C-reactive protein (CRP), antioxidant marker (FRAP), Copper, zinc, manganese, iron were analyzed in blood. Firstly, we evaluated the content of trace elements (Cu, Zn, Fe, Mn) in the Trema guineensis leave extracts. The ethanolic extract was richer in Cu and in Fe. Then TGE (200 mg/kg), drugs references (oligosol Cu/Zn: 0.50 mg/mL and oligosol Mn: 0.59 mg/mL) were administered to rats (N = 6) by intraperitoneal (0.5 mL). One hour after drug treatment all animals were injected with 0.2 mL of 1% suspension of carrageenan in normal saline into the subplanter region of left hind paw to induce inflammation and oxidative stress. Trace elements (Cu, Zn, Mn, Fe) were measured by flame atomic absorption spectrophotometer 5 hours later and compared to healthy controls (NaCl 0.9%). Mn, Cu, Fe and Zn concentrations varied significantly among the different groups. The ethanolic extract administering and the standard drug favored obtaining better trace elements contents. The CRP and oxidative stress by FRAP test in animals received carrageenan had confirmed its role in the installation of both pathologies in vivo. This analysis showed enough the plant extracts possess the anti-inflammatory and antioxidant activities. The analysis of iron showed that both extracts are rich in nonheminic iron.

Keywords: trema guineensis, oligosol, trace elements, carrageenan, Côte d’Ivoire.