EVALUATION OF IN-VITRO STUDIES OF ANTI-UROLITHIATIC ACTIVITY BY ETHANOLIC EXTRACT OF AMARANTHUS SPINOSUS

P. Sailaja*, Y. Prapurnachandra, K. Naga Jaya Varshini, M. Kavya, M. Dharani, T. Kaniha and M. Suman

ABSTRACT

This study evaluates the anti-urolithiatic activity of ethonolic extract. Urolithiasis occurs when stones exit the renal pelvis and enter the urinary collection system, including the ureters, bladder, and urethra. Amaranth spinosus belongs to the family Amaranthaceae, is the most widely grown leaf vegetable in Kerala and Tamil Nadu. The succulent stem and leaves are high in iron, calcium, vitamins A, and C. Both leaf and grain types play an important role in combating malnutrition among the poor. The present study was aimed to investigate the effect of Ethanolic extract prepared from Amaranthus spinosus leaves on the nucleation and aggregation of CaOx crystals. Nucleation is the most critical step in the process of stone formation, which begins with the combination of stone salts in the solution into loose clusters that may increase in size by adding new components. Based on the results obtained in this study, Amaranthus spinosus and showed maximum inhibition and the significantly highest inhibition was shown at 1000μg/mL, 79.96±1.48.

Keywords: Amaranth, urolithiatic activity, nucleation, renal stones.