STUDIES OF ANTIBIOTICS RESISTANCE OF PSEUDOMONAS AERUGINOSA FROM CLINICAL AND ENVIRONMENTAL (WATER AND SOIL) SAMPLES.

*Ogunnusi Tolulope Adeola and Adeyinka Rilwan Babatunde

ABSTRACT

This study was carried out to investigate the antibiotic resistance pattern of some Pseudomonas aeruginosa isolates from clinical and environmental (water and soil) samples. The isolates from the environmental samples were identified using the standard cultural, morphological and biochemical characteristics. A total of 47 bacteria were isolated, 37 were Gram negative and out of these, 4 were Pseudomonas aeruginosa, one isolate was from water and three from soil samples. Four clinical isolates were also used. P. aeruginosa was resistance to Nalixicidic acid, nitrofurantcin, cotrimoxazole, amoxicillin, tetracycline, augmentin and ceftazidime while sensitive to gentamicin and ofloxacin. The zone of inhibition of P. aeruginosa from blood sample was highest with ofloxacin (30 mm) followed by sputum and urine samples of 29m each. For the environmental samples, P. aeruginosa from water and from one of the soil samples had the highest zones of inhibition of 30mm with ofloxacin. There is a need to study the antibiotic resistant pattern so as to be able to counteract emerging resistant patterns of P. aeruginosa from different sources.

Keywords: Pseudomonas aeruginosa, resistance, antibiotics, environmental, clinical isolates.