HESPERETIN ENCAPSULATED PLGA NANOPARTICLES: SYNTHESIS, CHARACTERIZATION AND EVALUATION OF ITS IN-VITRO ANTIOXIDANT POTENTIAL AS WELL AS IN VIVO TOXICITY IN SWISS ALBINO MICE

Dibyendu Giri, Angsuman Das Chaudhuri, Anirban Majumder, Ahana Sinha, Subhabrata Das, Tanmoy Maity, Aranya Ghosh, Gouri Karan, Mrinmoyee Modak and Dr. Sujata Maiti Choudhury*

ABSTRACT

Hesperetin (HSP), a bioflavonoid, exhibits wide range of biological and pharmacological activities. It could be used as a prospective therapeutic agent by increasing its bioavailability and hydrophilicity. The aim of the current work is to fabricate hesperetin encapsulated PLGA nanoparticles and to investigate its toxicological effects as well as to assess its in-vitro antioxidant capacity. To address these objectives an environmental-friendly HSP loaded poly (lactic-co-glycolic acid) (PLGA) nanoparticles (PLGA-HSP NPs) was synthesized. DLS, UV-Vis studies were used to evaluate the properties of nano-hesperetin. The sub-acute toxicity of PLGA-HSP NPs was evaluated at different dose levels in Swiss albino mice after 28-days intraperitoneal administration. Hepatic and renal biomarkers of PLGA-HSP NPs treated mice were not altered significantly compared to free drug and control animals. The effects of nano-hesperetin as an antioxidant agent were also investigated. When PLGA-HSP NPs were compared to conventional ascorbic acid, they exhibited significantly greater free radicals scavenging action than HSP. In-vitro nitric oxide, superoxide, lipid peroxidation and hypochlorous acid scavenging tests revealed the potent concentration-dependent scavenging ability of PLGA-HSP NPs. In conclusion, it may be stated that PLGA nanoparticles encapsulated hesperetin is a non-toxic nano-conjugate and possesses a potent in-vitro antioxidant efficacy.

Keywords: Hesperetin; PLGA; Toxicity study; In vitro antioxidant activity; Swiss albino mice.