EUROPEAN JOURNAL OF
PHARMACEUTICAL AND MEDICAL RESEARCH

( An ISO 9001:2015 Certified International Journal )

An International Peer Reviewed Journal for Pharmaceutical, Medical & Biological Sciences

An Official Publication of Society for Advance Healthcare Research (Reg. No. : 01/01/01/31674/16)

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ISSN 2394-3211

Impact Factor: 7.065

ICV - 79.57

Abstract

ANTI-ARTHRITIC, CHONDROPROTECTIVE, AND CHONDROGENIC POTENTIAL OF NUTRITIONAL FORMULA IN MONOIODOACETATE-INDUCED OSTEOARTHRITIS ANIMAL MODEL

Dr. Shashi Kumar, *Dr. Gayatri Ganu, Dr. Dheeraj Nagore, Susheel Joshi and Dr. Vaishali Undale

ABSTRACT

Introduction: Osteoarthritis (OA) is a degenerative joint disorder hallmarked by progressive cartilage loss,
osteophyte formation, subchondral bone sclerosis, and synovial inflammation. These pathologic processes
ultimately manifest as joint pain, structural deformities, and functional impairment. This preclinical study aimed to
evaluate the anti-arthritic activity and chondrocyte regeneration potential of nutritional formula supplements in a
monosodium iodoacetate-induced (MIA) rat model of osteoarthritis. Materials & Methods: In the preclinical
study thirty-two Wistar rats were randomly divided into four groups, 8 rats in each group. Rats from sham control
(SC) and disease control (DC) groups received oral Carboxymethyl cellulose (CMC) 1%, while oral Indomethacin
(2 mg/ kg) in 1% CMC was administered to rats from PC group. The test group received an oral nutritional formula
(218.24 mg/kg) in 1% CMC for 28 days. Results: Preclinical study of nutritional formula demonstrated reduced
swelling and improved mobility within 7 days of treatment. By day 28, significant anti-inflammatory effects were
observed, evidenced by lowered COMP, MMP-13, and TNF-alpha levels. Radiological and histopathological
analysis supported these findings, indicating less degeneration in the nutritional formula group compared to
controls, suggesting potential for regeneration and chondroprotection. Conclusion: The preclinical findings
conclude that nutritional formula exhibits multifaceted benefits, including anti-arthritic and anti-inflammatory
effects, offering a promising alternative for knee osteoarthritis management. Additionally, nutritional formula
shows promise in promoting joint cartilage regeneration, although further investigations are warranted to assess its
chondrocyte protective effect in humans.