ELECTROANALYTICAL METHOD FOR SIMULTANEOUS ESTIMATION OF FOLIC ACID AND FERROUS ASCORBATE FROM PHARMACEUTICAL FORMULATION
Pralhad Rege* and Charuta Shrotri
.
ABSTRACT
A simple, specific, accurate, precise and reproducible method has been developed and validated for the
simultaneous determination of Folic acid and Ferrous Ascorbate using Differential Pulse Polarography (DPP)
technique. Quantification of Folic Acid and Ferrous Ascorbate was done in Acetate buffer pH 4.7 respectively
using 1M KCl as a supporting electrolyte. Both Folic Acid and Ferrous Ascorbate exhibit reduction cathodic peak
in given respective pH with peak potential (Ep) as -0.85V for Folic Acid and -1.281V for Ferrous Ascorbate vs.
S.C.E. Linearity of the method was found to be in the concentration range of 0.99 μg/mL to 6.54 μg/mL for FA and
1.98 μg/mL to 13.08 μg/mL for FEAS with correlation coefficient greater than 0.999 for both the analytes The
parameters were used for method validation are linearity; accuracy, precision, LOD and LOQ. Proposed method
was successfully applied for routine quality control analysis and simultaneous determination Folic Acid and
Ferrous Ascorbate in combined drug formulation.
Keywords: Differential Pulse Polarography (DPP), Folic Acid (FA), Ferrous Ascorbate (FeAs), Acetate buffer.
[Full Text Article]
[Download Certificate]