DETECTION OF THE ABILITY OF MANNOPROTEIN EXTRACTED FROMSACCHAROMYCES CEREVISIAETO INHIBIT THE ABILITY OF SOME SPECIES OF BACTERIA ISOLATED FROM DIFFERENT INFECTION SOURCES TO PRODUCE VIRULENCE FACTORS

Ashwaq Muthanna Wahib* and Mohammed Nadhir Maaroof

ABSTRACT

The study included (60) bacterial isolates from the total number of samples (100) samples, collected from different sources of infections and at different ages including both sexes, collected from patients with wound, burn and urinary tract infections. The isolates were diagnosed based on culture, microscopic and biochemical tests. Two isolates of Saccharomyces cerevisiae were selected for the production of mannoprotein .Mannoprotein was used in conjunction with urease, hemolysin and biofilm virulence media to demonstrate the effect of mannoprotein in preventing the ability of some pathogenic bacteria such as (Staphylococcus aureus, Escherichia coli, Proteus mirabilis, Klebsiella pneumonia) to product virulence factors. The results of the current study showed the ability of mannoprotein extracted from baker's yeast of the second and third types to prevent Staph. aureus bacteria from product the enzyme hemolysin. As for the bacterial isolates (K.Pneumoniae) under study, their ability to produce the virulence factor (urease) and (biofilm) was tested. The isolates under study were tested for the production of the enzyme urease. The results of the current study showed that the bacterial isolates did not produce the virulence factor (urease) for both types of mannoprotein due to its effect on preventing the bacteria from product the virulence factor at all concentrations. The ability of bacteria to product the virulence factor (biofilm) is considered a qualitative test to detect the ability to form biofilms based on the thickness and density of the biofilms associated with the inner wall of the test tube. Through the results obtained, the results of the study showed that the first isolate of bacteria (K.Pneumoniae) did not produce the virulence factor for the two types of mannoprotein used. As for the second isolate of bacteria, it was not productive of the virulence factor for the type of mannoprotein extracted from the first isolate used in neutralization and produced it when using the mannoprotein extracted from the second isolate and for all concentrations. Bacterial isolates (E.coli) Two isolates of the bacteria under study were used to detect the ability of mannoprotein to prevent bacteria from product (biofilm). The bacteria did not product the virulence factor due to their inability to produce the biofilm formation factor due to the effect of mannoprotein on preventing bacteria from producing the virulence factor. This is for the first bacterial isolate and for the two types of mannoprotein and at all concentrations. As for the second bacterial isolate, it produced the cell membrane factor when using mannoprotein from the first yeast isolate. However, when using mannoprotein from the second yeast isolate, it did not produce the virulence factor. As for Proteus bacteria, the first isolate of (P.mirabilis) bacteria was unable to product the virulence factor urease for the mannoprotein extracted from both yeast isolates for the first concentration only (100%) of mannoprotein, while the concentration (75%) and (50%) were ineffective in preventing the bacteria from product the virulence factor urease, and thus the bacteria were able to produce the virulence factor. As for the second isolate of (P.mirabilis) bacteria, it was unable to product the virulence factor urease for the mannoprotein of both yeast isolates at the concentration (100%) and (75%), while the concentration (50%) and as for the virulence factor biofilm, the first isolate of Proteus bacteria was unable to product biofilm in the presence of the mannoprotein extracted from both yeast isolates for the first concentration only (100%) of mannoprotein, while the concentration (75%) and (50%) were ineffective in preventing the bacteria from product biofilm. As for the second isolate of Proteus bacteria, it was unable to Biofilm product for mannoprotein of both yeast isolates at concentrations of (100%) and (75%), while concentration of (50%) was ineffective in neutralizing bacteria.

Keywords: Mannoprotein, Saccharomyces cerevisiae, Biofilm, urease, Hemolysin.