ISOLATION AND IDENTIFICATION OF PATHOGENIC BACTERIA IN DIABETIC FOOT ULCER (DFU) PATIENTS

Acharya Nagarjun Pyde*

ABSTRACT

The aim of his study was to isolate and identify the pathogenic bacteria in patient suffering from Diabetic foot ulcer (DFU) and to determine the antibiotic susceptibility pattern of the isolates. Specimen was cultured using optimal aerobic and anaerobic microbiological techniques. Identification was done by using gram staining and other biochemical methods. Antibiotic susceptibility pattern was done by using Kirby Bauer Disc Diffusion method. We further detected Methicillin resistance Staphylococcus aureus (MRSA) by using oxacillin disc, and extended spectrum betalactamase (ESBL). Twenty five organisms were isolated aerobically, out of which 10 (40%) were gram negative and 15 (60%) were gram positive. Among the gram negative isolates Pseudomonas were 8(32%) and Klebsiella 2(8%). Clostridia species was the only anaerobic bacteria which were identified. Most of Diabetic foot infection (DFI) specimens were monomicrobial infection and Predominent bacteria were S. aureus. Imipenem was sensitive to most of the isolates and we recomonds to be started empirically based on the clinical sign of the infection.

Keywords: Diabetic foot ulcer, Diabetic foot infection, Antibiotic susceptibility pattern, Aerobic, Anaerobic.