GENOTOXICITY OF BLOXIVERZ (NEOSTIGMINE METHYLSULFATE) ON BONE MARROW CELLS OF MOUSE

Dr. D. Rathna Prabha* and Dr. M. Ganga Raju

ABSTRACT

Neostigmine is used in post operational care to relieve the patient from anaesthetic effects. Today it is the most common agent used for the reversal of effects of other agents used for neuromuscular blocks, like Suxamethonium chloride, Atracurium etc. In our study, in vivo experiments were conducted on bone marrow of Swiss albino mice to assess the genotoxic effects of the drug on somatic chromosomes and mitotic index. Human therapeutic doses were converted to mice body weight basis ranging from minimum to maximum (2, 8 &20μg.) Experiments were conducted in single and cumulative dose series for 24, 48 and 72hr period. Slides were prepared from bone marrow of control and treated mice by following standard air drying technique and stained with 2% Giemsa. Mitodepression induced by Neostigmine is more in cumulative when compared to single dose series. In both series this effect is more in 24 hr period without any statistical significance. There is a gradual decrease after 48 and 72 hr period. The drug did not induce chromosome aberrations for all periods and doses to a significant extent. The observations indicate that Neostigmine is not genotoxic to bone marrow cells of mouse.

Keywords: Neostigmine, Genotoxicity, Chromosome aberrations, Cytogenetic protocols.