STUDY OF IMMUNOMODULATORY ACTIVITY OF ETHANOLIC EXTRACT OF THESPESIA POPULNEA BARK IN WISTAR RATS

Sanjiv Yadav*, Dr. Bipika Rajbanshi and Lalit Kumar Shah

ABSTRACT

In the present research immunomodulatory acitivity of Ethanolic extract of Thespesia populnea bark in wistar rats were studied. Initially drug was extracted with sufficient quantity of ethanol. The extract was tested for acute toxicity studies as per procedure given in OECD[61] guidelines. Albino Wistar rats, which were procured from central animal house of the Institute were used to carry out the experiment. Delayed type hypersensitivity(DTH) response, Humoral antibody (HA) titer, Total leukocyte count, Differential leukocyte count were determined. The test drug was compared with the standard drug Levimasole(50 mg/Kg). The ethanolic extract of Thespesia populnea bark in two different dose 200mg/kg and 400mg/kg were tested for their Immunomodulatory action, out of which the higher dose of 400mg/kg showed statistically significant Immunomodulatory activity.

Keywords: Thespesia populnea bark, Delayed Type Hypersensitivity (DTH), Total Leukocyte Count, Levimasloe, Wistar rats.