GREEN SYNTHESIS OF SILVER NANO PARTICLES BY USING CELASTRUS PANICULATUS LEAF, SEED EXTRACTS, CHARACTERIZATION AND ANTIBACTERIAL ACTIVITY

Soppari Pavan Kumar and Bukya Ramadevi*

ABSTRACT

Nanoparticle research is a promising field due to its diverse biological applications. Green synthesis, a cost-effective and sustainable method, involves synthesizing nanoparticles from organisms like fungi, algae, yeast, bacteria, and microbial enzymes, resulting in significant outcomes. Our study's primary goal is to investigate the process of synthesizing silver nanoparticles using Celastrus paniculatus leaf and seed extracts using environmentally friendly methods. The produced silver nanoparticles (AgNPs) from leaf (AgNPs-L) and seed (AgNPs-S) extracts were analyzed using Ultraviolet-Visible spectroscopy, and Fourier-transform infrared (FTIR). X-ray diffraction analysis (XRD), scanning electron microscopy (SEM) and also evaluated their antibacterial efficacy against three distinct strains. The UV-visible spectroscopic examination of the synthesized AgNPs-L shows maximum absorption peak at 419 nm and AgNPs-S at 425 nm at 423 nm, confirming the existence of surface plasmon resonance. The X-ray diffraction examination unveiled the crystal structure of the produced AgNPs, SEM confirms the particles sizes ranges 40nm to 150nm. S.aureus (MTCC-96), E.coli (MTCC-443) showed zone of inhibition (ZOI) of 21±0.60 mm and 12.6±0.81, with 25 and 10 μg/ml concentration of AgNPs-L which is maximum as compare AgNPs-S (19.13±0.30) and (10.63±0.37). Hence, the AgNPs generated via the process utilizing leaf extract exhibited strong antibacterial activities than AgNPs-S generated by seed extracts.

Keywords: Antibacterial; Celastrus paniculatus; Green synthesis; SEM; Silver nanoparticles.