SPECTRAL PROPERTIES, DNA STUDIES OF IRON(II) COMPLEXES OF ISONICOTINOYL HYDRAZONES

A. Suseelamma*, K. Raja and K. Hussain Reddy

ABSTRACT

A series of novel iron (II) complexes of general formula of Fe(L)2 [L=isonicotinoyl hydrazones) have been synthesized and characterized based on elemental analysis, molar conductivity, magnetic susceptibility measurements, infrared and electronic spectroscopy. Electrochemical behaviour of these complexes is investigated by cyclic voltammetry. The DNA binding constants Kb of the complexes are determined systematically with spectrophotometric titrations by using Calf Thymus DNA (CT-DNA). The changes in absorption spectra complexes upon addition of DNA suggest strong π- stacking interaction between the complexes and DNA base pairs. Cleavage activities of these complexes have been investigated on double stranded pBR322 plasmid DNA by gel electrophoresis in the absence and in presence of oxidant. The complexes behave as efficient chemical nucleases with hydrogen peroxide activation in the presence of reductant (DTT).

Keywords: iron (II) complexes, hydrazones, DNA binding, Nuclease activity.