NOVEL UV-SPECTROPHOTOMETRIC METHODS DEVELOPMENT AND VALIDATION FOR THE ESTIMATION OF VALACYCLOVIR IN BULK AND PHARMACEUTICAL FORMULATION

Suresha D. N.*, Prem H. N., Ashritha R., Chandan Gowda N. N., Hafnas C. M. and Harshini K.

ABSTRACT

A Novel, simple, accurate and precise Zero order derivative spectroscopic method was developed and validated for the estimation of valacyclovir in bulk and pharmaceutical formulation. The stock solution was prepared by weighing 100 mg of standard valacyclovir in 100 ml volumetric flask with Methanol. The stock solution was made to produce 1000 μg/ml with methanol. Further dilutions were prepared as per procedure. The drug solution showed the maximum absorbance at 254 nm. The linearity was found in the concentration range of 2-12 μg/ml. The correlation coefficient was found to be 0.999. The regression equation was found to be Y=0.043x+0.001. The method was validated for linearity, accuracy, precision, ruggedness, robustness, LOD and LOQ. The LOD and LOQ for estimation of valacyclovir were found to be 0.02738 μg/ml and 0.08298 μg/ml respectively. Recovery of valacyclovir was found to be in the range of 99.62-100.62 %. Proposed method was successfully applied for the quantitative determination of valacyclovir in bulk and pharmaceutical formulation.

Keywords: Valacyclovir, Zero order UV- Spectroscopy, Methanol, Accuracy.