DEVELOPMENT AND VALIDATION OF RP-HPLC METHOD FOR SIMULTANEOUS ESTIMATION OF METFORMIN AND EVOGLIPTIN IN PHARMACEUTICAL DOSAGE FORM

*Prof. Tejas J. Sharma, Lakhan S. Harkut, 3Prof. Shubham P. Kokate, 4Prof. Abhijit S. Dongardive, 5S. K. Bilal S. K. Yusuf and 6Ms. Sanjivani A. Chankhore

ABSTRACT

A Simple, Accurate, Precise method was developed for the simultaneous estimation of Evogliptin and Metformin in pharmaceutical dosage form. Chromatogram was run through ACCLAIMED mix mode HILIC-1; 5μ, 150 X 4.6mm. ID. Mobile Phase containing Ammonium acetate and acetonitrile (70:30 v/v) as a mobile phase was pumped through column at a flow rate of -1mL.min-1. Room temperature was maintained. Optimized wavelength of Metformin and Evogliptin was 205 nm. Retention time of Evogliptin and Metformin were found to be 4.93 min and 8.42 min. % RSD of the Evogliptin and Metformin were found to be 0.43 and 0.92 respectively. LOD, LOQ values are obtained from equation of Evogliptin and Metformin were 1.34, 0.75 & 4.46, 2.50 respectively. linear regression observed for EVO and MET. As resulted, they were y = 73859x + 44465 and y = 64111x + 19284, respectively.

Keywords: Method Development, Validation, Metformin, Evogliptin, RP-HPLC.