TRIAL FOR PREPARATION OF COMBINED INACTIVATED RVF AND SHIPPING FEVER VACCINE IN SHEEP

Mahmoud T. A. Ismail*, Fatma F. Ibrahim, Wafaa S. Abd El-Moneim, Ahmed F. Soudy, Mohamed Hassan Atwa and Diana Mohammed Abulmagd

ABSTRACT

Preparation and evaluation of a combined inactivated Rift Valley fever (RVF) and Shipping fever (SF) vaccine adjuvanted with MontanideTM ISA 206 was the aim. Single RVF vaccine and single SF vaccine were prepared with the combined one. These vaccines were sterile; safe and evaluated in 4 groups of sheep. The groups were vaccinated as follow; the first with RVF, the second with SF, the third with the combined vaccine, but the fourth was kept as a negative control. Each vaccinated group received two doses of its vaccine (1ml/dose); 1 month apart. The vaccinated groups showed raised immunity from first vaccination. SNT microtiter and ELISA techniques showed highest RVF antibody titers induced by RVF vaccines at the 3rd month post first dose keeping the protective level till the 12th month. ELISA for SF vaccines showed the highest antibody levels against P. multocida (A, B6) and M. hemolytica (A) at the 4th month post first vaccination. But the 5th month was the highest for P. multocida (D) and P. trehalosi (T). There was no significant difference for the same antigen in the different vaccinated groups, but for them and the control group a significant difference was noticed. For the protection rate against P. multocida (A, B6 and D), a passive mouse protection test was used. The protection rate for A and B6 was 98%, and D was 93%. Concisely, the prepared combined inactivated RVF and SF vaccine is a potent providing sufficient protectivity against both diseases, relieving stressors on animals and workers.

Keywords: RVF, ELISA, Pasteurella, Mannheimia, Vaccine, Shipping fever.