"HEPATOPROTECTIVE EFFECTS OF THE AQUEOUS EXTRACT OF CLOVE (SYZYGIUMAROMATICUM) AGAINST PARACETAMOL-INDUCED HEPATOTOXICITY AND OXIDATIVE STRESS IN RATS"

Mahdi M. Thuwaini, *Mohammed Abdul-Mounther and Hanaa S. Kadhem

ABSTRACT

Hepatoprotective activity of aqueous extract of clove (Syzygiumaromaticum) in albino rats was observed against controversial hepatotoxicity effects of paracetamol(PCM) induced liver toxicity in rats. Paracetamol was administered to induce hepatic damage in Wistar albino rats. 100 and 200 mg/kg doses of clove extract and were used as treatment groups. In present study, the effects of clove extract [100 mg per kg B.W. and 200 mg/kg.B.W.] as alone. On PCM-induced hepatotoxicity were examined. Rats were divided haphazardly into six groups containing 6 rats each. The control group received distal water (D.W.). Other groups were treated with PCM (600mg/kg) alone, (600mg/kg PCM + 100mg/kg clove extract), and (600mg/kg PCM + 200mg/kg extract) respectively for 4 weeks. The blood samples were analyzed for biochemical labels of hepatic injury and tissue samples were subjected for assessing of liver antioxidants and histopathological studies. Analysis of the treated rats with PCM (600 mg/kg) showed Paracetamol induced male rat hepatotoxicity represented by significant decline in the serum total albumin (P< 0.05). Conversely, the study declared significantly increment (P< 0.001), bilirubin, ALT, AST and ALP as shown in group2 (induction group) in compared with group1 (control group). While, simultaneous administration of clove aqueous extract (100mg/kg and 200mg/kg) with paracetamol, was displayed significantly attenuated the adverse changes in the serum total albumin, bilirubine, ALT IU/L, AST, and ALP. The histopathological examination in the liver of rats also encouraging that clove extract markedly diminished the toxicity of PCM and keeps the histoarchitecture of the liver tissue to near normal. Hence, the results postulate that clove extract acts as a potent hepatoprotective agent against PCM induced hepatotoxicity in rats.

Keywords: Hepatotoxicity, Syzygiumaromaticum, Wister rats, Paracetamol, Serum liver enzymes.