IN-VITRO ANTI-OXIDANT AND ANTI-DIABETIC ACTIVITIES OF ETHANOL EXTRACT OF CITRUS LIMON

Dr. Famobuwa O. E.*, Akinsiku R. O., Amuho U. E., Adekunbi E. A. and Ogungbemi T. S.

ABSTRACT

Citrus limon is a medicinal plant containing valuable bioactive compounds that functions as antioxidants, combating oxidative stress. It holds potential as an alternative treatment for various medical conditions like diabetes. This study was aimed at the evaluating the in-vitro antioxidant and anti-diabetic activities of the ethanol extract of Citrus limon. The extract was analyzed for its potential antioxidant activities using Ferric reducing antioxidant assay (FRAP) and DPPH radical scavenging activity. The anti-diabetic activities of the extract were determined using α-amylase inhibition assay and β-glucosidase inhibition assays. The FRAP of the ethanoic extracts was 9.68 mgFe2+E/100g while the maximum DPPH inhibition activity of 52.83% was reported at a concentration of 0.4 mg/ml and the minimum inhibition of 29.89% was reported at a concentration of 0.1 mg/ml. The α-amylase inhibition activity of the extract shows a maximum and minimum inhibition of 35.87% and 18.24% at a concentration of 0.2 mg/ml and 0.8 mg/ml respectively. The β-glucosidase inhibition activity of the extract shows a maximum and minimum inhibition of 60.92% and 23.11% at a concentration of 0.8 mg/ml and 0.2 mg/ml respectively. This result suggests the potential use of this plant species as a source of natural antioxidant and also in the management of diabetes, through its inhibitory effect on the activity of β-glucosidase.

Keywords: Citrus limon; anti-oxidant; anti-diabetic; ?-glucosidase.; ?-amylase.