EVALUATION OF ANTIDIABETIC AND ANTIOXIDANT POTENTIAL OF STEM EXTRACT OF CARISSA CARANDAS

Kirti Yadav, Pinki Jangra, Komal Sharma and Neha Yadav*

ABSTRACT

Carissa carandas Linn., also referred to as the Karonda christ hawthorn since it grows naturally in bushes. In India's tropical and subtropical Adriatic region, it is only occasionally grown. It is a popular medicinal herb in numerous indigenous medical practises, notably Unani, Ayurveda, and homoeopathy, and is utilised frequently by Indian tribal people. It collects little berries that are widely used to season and preserve Indian pickles and spices. In addition to Karonda karonda (Devanagari: karamardaka (Sanskrit), Koromcha (Bengali), Christ's thorns (South India), vakkay (Telugu), kilaakkaai (Tamil), and Karja tenga (Assamese), the prickly plant is also known by other names.), among others. The study focused on investigating the antidiabetic and antioxidant activities of Carrisa Carandas Linn. through the use of ethanol stem extract. Preliminary studies and GC-MS study revealed the presence of various phytochemical group like carbohydrates, amino acids, alkaloids, terpenoids, flavonoids, tannins and phenols. For antidiabetic activity, the extract demonstrated potent inhibition of α amylase and α glucosidase enzymes, with IC50 values of 47.12 μg/ml and 49.15 μg/ml, respectively. Regarding antioxidant activity, the extract displayed strong scavenging effect on DPPH free radical, with an IC50 value of 414.228 μg/ml, while standard antioxidant BHT had IC50 value of 50.173 μg/ml, hydrogen peroxide scavenging activity, with an IC50 values 493.656 μg/ml, while ascorbic acid had an IC50 value of 55.789 μg/ml, The extract of lipid peroxidation assay with an IC50 value of 7.786 μg/ml, while BHT had IC50 value 3.198 μg/ml. In conclusion, these findings suggest that the ethanol extract of stem of Carissa carandas Linn possesses antidiabetic and antioxidant activities. However further studies are very much needed to explore the mechanism of action and other potential effects using animal studies.

Keywords: Carissa carandas; Antidiabetic activity; Antioxidants; Cytotoxicity.