DEVELOPMENT OF UV-VISIBLE SPECTROPHOTOMETRIC METHOD FOR ESTIMATION OF CARVEDILOL IN BULK AND PHARMACEUTICAL FORMULATION

B. Satya Prasad*, J. N. Suresh Kumar, B. Deepak, B. Mahesh Naik, I. V. S. Lasya, M. Chandra Varshini and K. Ramya

ABSTRACT

A clear, highly accurate and sensitive spectrophotometric techniques has been developed to measure carvedilol in pharmaceutical formulation and in bilk drug. In this study methanol was determined to have the highest assay sensitivity and sufficient drug solubility. In the 200-400nm wavelength range, the absorbance of carvedilol was recorded at 245nm. In the concentration range of 10-60ug/ml, beer’s law was followed. Additionally, the correlation coefficient slope, intercept were also calculated. The medication in both its pure and tablet dose form has been successfully analysed using suggested method. The percentage recovery results indicate that the presence of common excipients has no effect on the procedure sensitivity and precision tests were used to validate the approach, demonstrating its applicability for routinely estimating carvedilol in pharmaceutical formulation and bulk. Carvedilol interacts with ninhydrin in the presence of a base producing a yellowish orange complex with a maximum fall in visible region. When the drug and chromogenic agent combine, a yellowish orange solution is produced, with the maximum absorbance being measured at 405nm. The UV spectrophotometric and colorimetric techniques were thoroughly tested in accordance with ICH guidelines and all the parameters were within the acceptable range with a correlation of 0.9925 and a% RSH of 0.6441. The linearity was evaluated and found to be between 100-600ug/ml. The techniques developed in this study offer a practical and precise means of estimating carvedilol.

Keywords: Carvedilol, UV-spectrophotometric estimation, Colorimetric estimation, Method development, Method validation.