EXTRACTION, EVALUATION AND IDENTIFICATIONOF APAMARGA EXTRACT ALONG WITH ISOLATION OF ACTIVE COMPOUND RESPONSIBLE FOR ANTI- INFLAMMATORY ACTIVITY

Adesh Mani Bajpai*, Dr. Abhinav Prasoon Mishra and Dr. C. S. Bhargav

ABSTRACT

This study investigates the anti-inflammatory properties of Achyranthes aspera, a widely recognized medicinal plant in the Amaranthaceae family. Traditional applications include treatment for various ailments such as dropsy, skin eruptions, and as an antidote for snake bites. The primary objective was to extract, evaluate, and isolate the active compounds responsible for the plant's anti-inflammatory effects. Extraction was performed using pet ether, chloroform, and ethanol, with the highest yield obtained from the ethanol extract, which also contained significant phytochemical constituents including carbohydrates, flavonoids, phenols, saponins, terpenoids, and tannins. Quantitative analysis revealed a total phenolic content of 0.935 mg/100 mg and flavonoid content of 0.900 mg/100 mg in the ethanol extract. The anti-inflammatory efficacy of both the ethanol extract and oleanolic acid was assessed through albumin denaturation inhibition and anti-proteinase assays. Results demonstrated significant inhibition rates, with oleanolic acid achieving 93.56% and 95.90% inhibition at doses of 80 and 100 μg/kg, respectively, alongside albumin denaturation inhibition rates of 85.48% and 93.65%.

Keywords: Anti-inflammatory, Phenolic, Flavonoid, Apamarga, Isolation, Dropsy, Antidote, Evaluation.