ANXIOLYTIC EFFECT OF MITRAGYNA INERMIS LEAVES EXTRACT IN MICE USING OPEN FIELD TEST

Toheeb Ayodeji Sodiq*, Abass Bolarinwa Dada, Miracle Oladoyin Ojedayo, David Izuchukwu Nnezianya, David Archibong Ephraim, Oluwafemi Emmanuel Akinyemi, Omonike Temiloluwa Olulana and Olaitan Ebenezer Oluwadare

ABSTRACT

Background: Anxiety is an emotion characterized by feelings of tension, worried thoughts, and physical changes like increased blood pressure. It' is considered a future-oriented, long-acting response that is broadly focused on a diffuse threat. Anxiety is distinct from fear, which is a present- oriented and short-lived response to a specific threat. Aim: This study aimed to evaluate the anxiolytic Effect of Mitragyne inermis Leaves. Method: The study analyzed the phytochemical content, toxicity, and anxiolytic effects of an aqueous extract. Alkaloids, flavonoids, tannins, and steroids were detected, known for their analgesic properties. Results: Acute toxicity tests showed an LD50 of 3808 mg/kg, indicating safety. A nxiolytic tests on mice revealed significant effects at 500 mg/kg and 500 mg/kg, with 1000 mg/kg performing comparably to Diazepam. Conclusion: These findings suggest that Mitragyne inermis leaves extract may serve as a natural alternative to conventional anxiolytic drugs, offering a promising option for anxiety management with a fewer side effect.

Keywords: Anxiety, Anxiolytic effect, Mitragyne Inermis, Open Field Test.