INVITRO ANTI UROLITHIATIC POTENTIAL OF SAUROPUS ANDROGYNOUS AND POLYALTHIA LONGIFOLIA

Meghana B P .*.*, Kruthika B C .., Sinchana D M . and Varsha C N

ABSTRACT

Despite advanc
es in modern medicine, the urolithiasis continues to be a source of concern for mankind, as there is
no effective treatment for kidney stones. In the present study we investigated antiurolithiatic activity of Sauropus
androgynous and Polyalthia longifolia against calcium oxalate precipitation. Both the extracts are dissolved in
water and the concentration of 100, 300, 500 a n d 7 0 0 µg/ml are obtained. A mixture of 1 ml of artificial urine and 0.5 ml
of extract solution is taken in the cell. A blank readin g is taken and then 0.5 ml of 0.01 M sodium oxalate solution
is added and immediately absorbance is measured for a period of the 10 minutes with 2 minutes interval at 620 nm.
The results of ethanolic plant extracts of Sauropus androgynous exhibits dose and time-dependent % inhibition. The inhibition started by 100μg\ml with 15.77% and maximum inhibition 700 μg\ml was observed at 37.2%. The results of ethanolic plant extracts of Polyalthia longifolia exhibits dose and time-dependent % inhibition. The inhibition started by 100μg\ml with 22.14% and maximum inhibition 700 μg\ml was observed at 64.22% respectively. Both the extract shows significant dose and time-dependent % inhibition. This study emphasized the need to carry out in-depth pharmacological evaluations of this property in other dissimilar models and further isolation of chemical constituents responsible for antiurolithiatic activity.

Keywords: Urolithiasis, Phyllanthaceae, Annonaceae, Calcium oxalate, Cystone, Renal calculi.