SUDAN DYES AS LIPID SOLUBLE ARYL-AZO NAPHTHOLS FOR MICROBIAL STAINING

Rutvi Patel, Clive Dadida, Kinsuk Sarker and Prof. Dr. Dhrubo Jyoti Sen*

ABSTRACT

Sudan stains and Sudan dyes are synthetic organic compounds that are used as dyes for various plastics and are also used to stain sudanophilic biological samples, usually lipids. Sudan I, Sudan II, Sudan III, Sudan IV, Oil Red O and Sudan Black Bare important members of this class of compounds. Sudan dyes have high affinity to fats; therefore they are used to demonstrate triglycerides, lipids and lipoproteins. Alcoholic solutions of Sudan dyes are usually used; however pyridine solutions can be used in some situations as well. Fat soluble Sudan dyes can easily stain the lipoprotein & phospholipid layer of bacterial cell to produce gram staining of gm+ve and gm-ve bacteria.

Keywords: Sudan stains, Aryl-azo naphthols, Lipids, Triglycerides, Lipoproteins, Phospholipids, Gram Staining.