IN VITRO ANTI OXIDANT ACTIVITY OF ETHANOLIC EXTRACT OF DIOSPYROS PANICULATA Dalz, BARK

Mithun K. Sebastian* and Shan P. Mohammed

ABSTRACT

Diospyros Paniculata is a tree which belongs to the family Ebanacea which has been used as a folkforic medicine in ayurveda and siddha. It has been commonly used in the treatment of ulcer, burns, poisoning etc. Here the study was conducted to evaluate the In vitro Anti oxidant activity of bark extract of diospyros paniculata by DPPH, Hydroxyl radical scavenging methods. The effect of the extract is assessed by the ability to scavenge DPPH radicals and Hydroxyl radicals. The results obtained indicate that the Ethanolic extract of Diospyros Paniculata (EEDP) has significant In vitro Anti oxidant activity (P<0.001).The IC 50 value of EEDP by DPPH method was found to be 100μg/ml as compared to standard which is 70μg/ml and Percentage Inhibition of Hydroxyl radical at dose 1000μg/μl of EEDP is 38.07% as that of standard which is 75.44%.

Keywords: Diospyros Paniculata, In vitro, Anti oxidant, DPPH, Hydroxyl radical scavenging.