THE EFFECT OF PARTIAL PURIFIED PLANTARICIN AGAINST URINARY TRACT INFECTION (UTI) INDUCED BY ESCHERICHIA COLI AND PROTEUS MIRABILIS IN EXPERIMENTAL RAT MODEL

*Rana Hussein Raheema and Abdul Karim Salim Mahood

ABSTRACT

The present study was purposed to assess the effect of plantaricin of L. plantarum in vivo by inducing uti in rats with Escherichia coli and Proteus mirabilis and to check out its effect on some biochemical and histopathological parameters. Fifty four rats were divided into five groups, Group (A): negative control (not infected group), Group (B): positive control (infected E. coli, P. mirabilis respectively and not treated group) for 2,5 and 10 days, Group (C): Animals were administrated orally 160 AU / ml of plantaricin for 14 days then infected with E.coli and P. mirabilis for 2,5 and 10 days, Group (D): infected with E.coli, P. mirabilis respectively and treated orally with 160 AU / ml of plantaricin for 14 days and Group (E): infected with E.coli and P. mirabilis respectively and treated with gentamicin 2 mg /ml. B.W.I.M for 14 day. At the end of experimental period, blood samples were taken to estimate the biochemical measurements such as serum creatinine and uric acid. Results showed an elevation of serum creatinine level in infected group with Escherichia coli and P. mirabilis compared with normal group. Result showed significant changes in serum uric acid and creatinine in infected and treated groups. Histological sections of kidney showed severe damage in infected groups compared with treated groups. It could be concluded that plantaricin of L. plantarum has potent protective role against UTI and appears by correction between biochemical parameters and histological studies.

Keywords: L. plantarum, E. coli, P. mirabilis.