ASSESSMENT OF ACUTE TOXICITY AND CYTOTOXICITY OF AQUEOUS EXTRACT (EA), ETHYL ACETATE FRACTION (FA) AND METHANOL FRACTION (FM) OF EUCALYPTUS CITRIODORA.

*Oungbé Monkoué Désiré, D’Almeida Marie-anne, Boga Gogo Lucien, Kouassi Konan, Djaman Alico Joseph and N’guessan Jean David

ABSTRACT

The objective of this study was to investigate the toxicity of E. citriodora. In order to evaluate the toxicity of E. citriodora, the acute toxicity of the aqueous extract, ethyl acetate fraction and methanol fraction of this plant on female rats and cytotoxicity test on NIT-1 cell line were investigated. A fixed large dose of 2000 mg/kg b.w. of aqueous extract, ethyl acetate and methanol fractions was administrated by a single oral gavage according to the OECD procedure. In 2 weeks, aqueous extract, ethyl acetate and methanol fractions did not significantly affect the weight gain, hematological and biochemical parameters of rats treated groups compared with the control group. Cytotoxicity effect of aqueous extract, ethyl acetate fraction and methanol fraction was estimated through a MTT assay. Cytotoxicity tests on NIT-1 cell line disclosed that concentrations of 10, 100 1000 μg/mL inhibited the growth of cells. The concentration inducing cell growth inhibition by about 50% (IC50) were 100 μg/mL. The results of cytotoxicity show toxic effect of E. citriodora extract and fractions on NIT-1 cells. While this work did not showed acute toxicity effect on NIT-1 cells.

Keywords: Eucalyptus citriodora; aqueous extract; ethyl acetate fraction, methanol fraction; cytotoxicity; oral acute toxicity; MTT assay.