A SELECTIVE UV SPECTROPHOTOMETRIC ANALYTICAL METHOD TO QUANTIFY THE LEVOFLOXACIN IN BULK SAMPLE.

Lonikar N. B.*, Baby Sudha Lakshmi P. and Mallikarjuna Gouda M.

ABSTRACT

Levofloxacin is a broad spectrum antibacterial agent and belongs to floroquinolones group. The analytical method developed for the quantification of levofloxacin in bulk sample showed absorbance maximum (λmax) of 291.2 nm in distilled water between 200 nm and 400 nm. Linearity study between 2.00 μg/ml to 10.00 μg /ml was found to be linear with regression equation of Y = 0.074*X +C; (r2 = 0.9999). The accuracy, precision studies showed that the recovery of drug from bulk sample and dosage form are highly accurate and precise with minimum error. The above analytical parameters indicated that the developed UV Spectrophotometric method for Levofloxacin was simple, accurate, precise and reproducible.

Keywords: Levofloxacin, Precession, linearity, regression equation, Absorbance maximum.