A STABLE HPLC BIOANALYTICAL METHOD DEVELOPMENT FOR THE ESTIMATION OF BUDESONIDE IN PLASMA
Lonikar N. B*, Mallikarjuna Gouda M, Baby Sudha Lakshmi, Ramakrishna Shabaraya A
ABSTRACT
In pharma industry the development of analytical method is very important in drug analysis, development and in
pharmacokinetics study of the drug. Hence in the present investigation the HPLC bioanalytical method of liquidliquid
extraction of drug from plasma is established and the linearity study of Budesonide was found in range of
50% to 150 % concentration, the regression equation was found to be Y = 22326X + 15677. The method checked
for Pression and % RSD was found to be 1.72 % and the accuracy was in the range 100.4 % to 101.1%. The limit
of detection and the limit of quantification were calculated by the non instrumental that is by equation mentioned in
the methodology. The LOD was found to be 191.3μg/ml and LOQ was found to 579.9 μg/ml. Thus the developed
bioanalytical HPLC is selective, stable and quantity the budesonide in plasma.
Keywords: Budesonide, nimusolide, Linearity, HPLC, Relative standard deviation.
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