DETECTION OF THE ANTIBACTERIAL ACTIVITY OF BACTERIOCIN FROM LOCAL ISOLATES OF PSEUDOMONAS AERUGINOSA MULTIPLE RESISTANT STRAINS AGAINST GRAM POSITIVE&GRAM NEGATIVE BACTERIA

Mais E. Ahmed* and Dr. Muna T. Al-Mossawy

ABSTRACT

The antibacterial activity of local isolates frome isolated from Baghdad, Iraq samples of different sources urine and wounds, ear&eye swab (25) strain of Pseudomonas aeruginosa (P1) (from wound infaction) multiple resistant strains that produced largest inhibition zone against the indicator strain was chosen for further study, and identified by morphological, physiological properties and antibiotic sensivity test were the neutralized Pseudomonas aeruginosa (P1) cell free supernatant of inhibited growth of a number of standard strains of pathogenic microorganisms bacteria Gve + and Gve- These isolates were screened for bacteriocin production using the agar well diffusion method. The isolates of P. aeruginosa were producer of bacteriocin with a wide inhibition range on growth of gram positive and negative pathogenic bacteria. P1 inhibited the bacterial growth of S. aureus, Bacillus subtilis, Streptococcus pyogens, isolates with a range of inhibition zone (15-21) mm. while inhibited the bacterial growth gram negative Klebsiella pneumonia, Salmonella typhai, Escherichia coli with a range of inhibition zone was (13- 22) mm.

Keywords: Pseudomonas aeruginosa, antibacterial activity, bacteriocin, pathogenic Bacteria.