EFFECT OF ALCOHOLIC SEED EXTRACT OF CAESALPINIA BONDUCELLA ON MORPHOMETRY OF ENDOCRINE BETA CELL IN NORMAL AND ALLOXAN INDUCED DIABETIC MALE ALBINO RATS

Vedavyasa Sagar, Subhas C. Bijjaragi and R. Nazeer Ahamed*

ABSTRACT

Present study was designed to evaluate the efficacy of seed extract of Caesalpinia bonducella a medicinal plant in healing and ameliorating pancreatic beta cell in alloxan induced diabetic male albino rats. The rats were divided into six groups - Normal-Control, Diabetic-Control, Normal+200mg ASECB, Normal+400mg ASECB, Diabetic+200mg ASECB and Diabetic+400mg ASECB. Rats made diabetic by intra peritoneal injection of alloxan. Oral administration of different doses of ASECB to normal and diabetic rats was conducted for a period of 14 days. Morphometry of beta cell and its nucleus was studied. Diabetic control group showed a significant (P<0.05) fall in cellular and nuclear diameter of beta cell, while the diabetic rats treated with different doses of ASECB showed a significant increase in cellular and nuclear diameter. In normal treated rats, the cellular and nuclear size of beta cell more or less similar to control rats indicating that the plant extract has no side effects. The higher dose of 400mg is more effective than 200mg. It is evident from the present histological study that ASECB can ameliorate and restore the cellular and nuclear diameter of beta cell to normalcy. Thus ASECB could serve as a good healing agent.

Keywords: Diabetes, Caesalpinia bonducella, Beta cells, Alloxan, Cellular, Nuclear.